Abstract

PONCE DE LEON, Patricia; FORESTO, Patricia  and  VALVERDE, Juana. Ascaris lumbricoides: Inhibitory capacity in relation to P₁ antigen. Acta bioquím. clín. latinoam. [online]. 2007, vol.41, n.2, pp.225-228. ISSN 0325-2957.

P Blood Group has a unique antigen: P₁. It was identified in various parasite species. The aim of this work was to determine A. lumbricoides´s Inhibitory Capacity in relation to P₁ antigen. The work was based on 11 parasite extracts (AE). The semiquantitative inhibition agglutination technique was used to assess the extracts´ Inhibitory Capacity in relation to P₁ antigen. Two series of anti- P₁ monoclonal antibody dilutions were prepared. PBS was added to the first series and an equal volume of AE to the second. Suspensions of P₁ red cells were used as a revealing system. Monoclonal antibody titres were determined in both series. The agglutination was considered significative when there was a difference of two or more dilutions between the titres. Eight of the 11 extracts studied presented P₁ epithopes. Inhibitory power of these 8 extracts was calculated and it was related with their protein concentrations to compare the Inhibitory Capacity among the extracts. The technique used made it possible to prove P System epithopes presence and to compare the extracts´ Inhibitory Capacity in relation to P₁ epithopes.

Keywords : Ascaris lumbricoides; inhibitory capacity; P₁ antigen.

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