Abstract

ZIMATH, Thaise; PINOTTI, Priscila; BERNARDON PRETTO GONCALVES, Juliana  and  CAMATI PERSUHN, Darlene. Biological variation in concentration of serum lipids. Acta bioquím. clín. latinoam. [online]. 2008, vol.42, n.1, pp.53-59. ISSN 0325-2957.

Biological variability is defined as a natural variation, with physiological occurence, independent of pre-analytical factors. The individual answer to the different stimulations to which the organism is subjected to results in degrees of biological variation that are change between subjects. This degree of variation must be quantified to help the laboratory results interpretation. The objective of this project was verify the biological variability in lipids determinations (total cholesterol, HDL cholesterol, LDL cholesterol, and triglycerides) in healthy subjects. Ten healthy individuals participated in this projetct. The samples were collected by venopunction, ten times, in three consecutive weeks. The serum obtained was analysed in automatized equipment Cobas Mira" (Roche) using commercial kits. Both individual and total variability coefficients found were similar to the literature. Total cholesterol (6.3%) and HDL-cholesterol (4.9%) showed the lowest variabilities, whereas triglycerides presented the biggest index (25%). In some patients the variation coefficient for any analyte was superior than the range previously described in the literature. New sample analyses must be performed when there is some discrepancy in clinical or biological interpretation between results.

Keywords : Lipids; Diagnostic use; Cholesterol; Triglycerides; Lipoproteins.

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