Abstract

IMPERIALE, Belén Rocío. Meta-analysis: drug-resistant tuberculosis diagnosis by non commercial amplification techniques. Acta bioquím. clín. latinoam. [online]. 2013, vol.47, n.2, pp.407-418. ISSN 0325-2957.

Due to the emergency of multidrug and extensively-drug resistant tuberculosis, molecular methods for a rapid detection of isoniazid (INH) and rifampicin (RIF) resistance are urgently needed. For that reason, the objectivesof this study were to asses through a meta-analysis the global accuracy and the utility of the home-made molecular methods based in PCR for INH and RIF resistance rapid detection from Mycobacterium tuberculosis clinical isolates. The articles were searched using Medline/PubMed, BioMedLib. The variability among different studies results and the diagnostic accuracy of the used methods were estimated by forest plot and summary receiver operating characteristic (SROC) curves performed with software Meta-DiSc. Fifteen studies were chosed: 1311 containing INH resistant and 953 RIF resistant isolates. The pooled sensitivity and specificity for INH resistance detection was 84.0% and 96.0% respectively, while 92.0% and 97.0% were the pooled values for RIF resistance detection. Besides, these methods showed a high diagnostic accuracy, with the area under the SROC curve >0.9. Due to the high sensitivity and specificity obtained with the home-made molecular methods, some of these tests could be applied for a rapid detection of M. tuberculosis drug resistance in clinical practice.

Keywords : Mycobacterium tuberculosis; Tuberculosis; Drug susceptibility; Rifampicin; Isoniazid; Molecular methods; Polymerase chain reaction; Rapid methods.

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