Abstract

LOPEZ MURUA, Gabriel; RACCA, Liliana  and  PONCE DE LEON, Patricia. Study of kinetics of sialic acid capture by muscle larvae of Trichinella spiralis. Acta bioquím. clín. latinoam. [online]. 2015, vol.49, n.2, pp.267-272. ISSN 0325-2957.

It has been reported that erythrocytes (RBC) which were incubated with infective larvae of T. spiralis exhibit higher aggregation than RBC Controls incubated with saline solution, indicating that the parasite captures erythrocyte sialic acid. The objective of this work was to study the kinetics of sialic acid capture by muscle larvae of T. spiralis using Digital Image Analysis. The work was performed with larvae concentrates of T. spiralis, which were incubated with an equal volume of O Group RBC in saline and enzymatic mediums (Treated RBC), for 120 minutes at 15 minute intervals. Digital Image Analysis was applied and the value of Isolated Cells Coefficient (ICC) was calculated. To analyze the effect of incubation time on the ICC values, an analysis of variance was used to design randomized complete blocks. The results showed that the average value of ICC, both saline and enzymatic mediums, varied significantly with incubation time. It was shown that the increase in the contact of the larvae with erythrocytes produces the decrease of globular sialic acid, which is reflected in higher ICC values. The experience would suggest that during their stay and viability in the trichina cysts, the larvae could be capturing muscle sialic acid in order to interfere and/or evade the host immune response.

Keywords : Trichinella spiralis; Kinetics; Sialic acid capture.

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