Abstract

OCHIUZZI, María E  and  RED DE MICOLOGIA CABA et al. Evaluation of the VITEK 2 system (AST-YSO1 cards) for antifungal susceptibility testing against different Candida species. Rev. argent. microbiol. [online]. 2014, vol.46, n.2, pp.111-118. ISSN 0325-7541.

The aim of this investigation was to evaluate the results of antifungal susceptibility for various Candida species using the Vitek 2 semi-automated system (AST-YSO1 cards, bioMérieux), and to compare them with those obtained by the CLSI (Clinical and Laboratory Standards Institute) broth microdilution reference method (Document M27-A3,2008). The essential agreement (EA) was > 90%, except for Candida glabrata against voriconazole (VCZ); and for Candida krusei against fluconazole (FCZ). The overall categorical agreement (CA) was > 90% when FCZ was evaluated and 89.5% at 24 h and 80.7% at 48 h for VCZ. The average time for obtaining results was 15.5 h. Minor errors were 7.8% at 24 h and 6.1% at 48 h for FCZ, and 10.5% at 24 h and 19.3% at 48 h for VCZ. There was only one very major error for FCZ against Candida parapsilosis and no major errors were observed. For amphotericin B, only three isolates showed MICs = 2 ?g/ml. The Vitek 2 system detected the MIC value for various Candida species and showed excellent agreement with the reference method proposed by the CLSI.

Keywords : Vitek 2 system; Susceptibility testing; Candida spp.

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