Abstract

RELLOSO, María S et al. Evaluation of mass spectrometry: MALDI-TOF MS for fast and reliable yeast identification. Rev. argent. microbiol. [online]. 2015, vol.47, n.2, pp.103-107. ISSN 1851-7617.  http://dx.doi.org/10.1016/j.ram.2015.02.004.

The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technique known as MALDI-TOF MS is a tool used for the identification of clinical pathogens by generating a protein spectrum that is unique for a given species. In this study we assessed the identification of clinical yeast isolates by MALDI-TOF MS in a university hospital from Argentina and compared two procedures for protein extraction: a rapid method and a procedure based on the manufacturer's recommendations. A short protein extraction procedure was applied in 100 isolates and the rate of correct identification at genus and species level was 98.0 %. In addition, we analyzed 201 isolates, previously identified by conventional methods, using the methodology recommended by the manufacturer and there was 95.38 % coincidence in the identification at species level. MALDI TOF MS showed to be a fast, simple and reliable tool for yeast identification.

Keywords : Yeast; MALDI-TOF MS; Identification.

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