Abstract

MESTRE, María Cecilia; LIBKIND, Diego  and  FONTENLA, Sonia. Comparison of culture conditions for isolation and count of soil yeasts from Nothofagus spp. (Fagaceae) native forest of Argentinean Patagonia. Bol. Soc. Argent. Bot. [online]. 2009, vol.44, n.3-4, pp.229-238. ISSN 1851-2372.

This research proposes the use of culture medium added with 25 >g/mL of Rose Bengal and 200 >g/mL of Chloramphenicol. Under these culture conditions the yeast counts were about 10³ CFU/g of soil (approximately 4% of detected microflora). Isolation with and without a previous enrichment step was compared. Isolated yeasts were classified into 7 groups based on morphological and physiological characteristics. 10 different banding patterns were observed using MSP-PCR technique, which indicate the presences of at least 10 species. Different banding pattern was observed between cultures with and without enrichment, this revealed the need of using both isolation techniques in a complementary way to recover strains inhibited or underestimated by direct non-enrichment methods.

Keywords : Yeast; Soil; Forest; Nothofagus; Culture media; Isolation medium.

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