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Revista de Ciencia y Tecnología

On-line version ISSN 1851-7587

Abstract

ARISMENDY, Ana María et al. Optimisation of the enzymatic hydrolysis of rice husk. Rev. cienc. tecnol. [online]. 2019, n.32, pp.1-10. ISSN 1851-7587.

The objective of this work was to find the optimal conditions of enzymatic hydrolysis of the rice husk pretreated with sodium hydroxide in an autoclave at 121° C. Screening design showed that all the selected variables (FPU / g substrate, pH, agitation, time, temperature and concentration of surfactant) were statistically significant. Then an optimization design was applied for the variables: pH, FPU and time, discarding the ones with the lowest level of significance. Finally, the best conditions found in the previous designs were (pH 5.0, 13 h, and 30 FPU / g of substrate) subsequently these conditions were applied in experiences to evalúate the influence of the increase ¡n hydrolysable cellulose, using 6%; 8% and 10% of cellulose available giving saccharification yields of 74%, 42% and 16%.

Keywords : Rice husk; Enzymatic hydrolysis; Lignocellulosic Biomass; Optimal Conditions; Bioethanol.

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