Abstract

BRACALE, Karen; GONZALEZ, Roschman  and  OSORIO-VEGA, Héctor. Morphological description of testate amoeba of the genus Arcella sp., Through the use of multiple techniques. Rev. cienc. tecnol. [online]. 2019, n.32, pp.1-10. ISSN 1851-7587.

In the research morphologically, amoebas of the genus Arcella sp are described by the use of culture techniques, scanning electron microscopy (SEM), and morphometry. For this, a sample of 5μl of these microorganisms was taken in a saline culture medium, previously standardized. Being an aqueous sample, a standardization of the method used for observation through the MEB using a low vacuum mode or environmental force. In this regard, the samples were fixed with 5μl of Karnovsky solution at 2% for 15 min and post-fixed with 5μl of 1% osmium tetraoxide solution for 15 min. Subsequently, a sample of the mixture was taken, which was placed in a copper grid previously covered with a nitrocellulose film and coated with a carbon film for greater mechanical strength and allowed to dry in an oven at room temperature for 20 min, once the sample was placed. Finally, it was observed in a scanning electron microscope in low vacuum mode (ESEM) and from the digitized images, the dimensions of the diagnostic characteristics of interest were obtained, with the help of the ImageTool 3.0 program. The results obtained from this study allowed to describe in a general way the diagnostic characteristics of a group of tested amoebas belonging to the genus Arcella sp.

Keywords : Amoebas; Arcella sp; Morphology; Cultures; Scanning electron microscopy..

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