Resumen

FAVERO, Paulo Roberto; SOARES LEONART, Maria Suely  y  DO NASCIMENTO, Aguinaldo José. Electrophoresis of erythrocyte membrane and hemolytic diseases caused by membrane abnormalities. Acta bioquím. clín. latinoam. [online]. 2004, vol.38, n.3, pp.313-317. ISSN 0325-2957.

The erythrocyte membrane contains a net of proteins, forming the cell backbone, and the integral proteins, immerging in the lipid bilayer, both important for the integrity maintenance and for the cellular activity. We studied 14 patient carriers of hemolytic diseases, assisted in the Centro de Hematologia e Hemoterapia do Paraná (HEMEPAR) in relation to control group of 20 healthy volunteers. Samples of venous blood were collected in citrate-phosphate-dextrose (CPD). The membranes were isolated [Dodge et al., Arch. Biochem. Biophys. 100:199, 1963], the protein concentration was determined [Lowry et al., J.Biol.Chem. 193: 265, 1951] and were submitted to the vertical electrophoresis (SDS-Page) [Laemmli, Nature 227: 680, 1970]. The values obtained in the control group (%; n = 10) were: spectrins = 29.67 ± 4.14; ankyrins = 3.97 ± 1.84; band 3 = 38.70 ± 4.96; band 4.1 = 6.79 ± 1.60; band 4.2 = 5.00 ± 1.43; band 4.5 = 1.89 ± 0.96; band 4.9 = 1.83 ± 1.22; band 5 = 6.54 ± 3.13; band 6.0 = 2.70 ± 1.24; band 7.0 = 2.36 ± 1.33. The mean value for the spherocytosis carriers was 24.4% for spectrin and 35.8% for band 3, (t tests; p <.0.05). The observation of blood spherocytes or elliptocytes, associated to an electrophoretic profile with deficiency of spectrin or band 3 suggests spherocytosis or hereditary elliptocytosis, respectively. Assimptomatic carriers of erythrocyte membrane molecular abnormalities can also be detected through SDS-Page.

Palabras clave : Red blood cell membrane proteins; Hereditary elliptocytosis; Hereditary spherocytosis; Red blood cell morphology; Hemolytic diseases.

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