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BAG. Journal of basic and applied genetics
versión On-line ISSN 1852-6233
BAG, J. basic appl. genet. vol.31 no.2 Ciudad Autónoma de Buenos Aires dic. 2020
http://dx.doi.org/10.35407/bag.2020.31.02.01
Articles
First karyotype report on Colocasia oresbia: a comparative cytogenetic study between two varieties
Primer reporte del cariotipo de Colocasia oresbia: un estudio citogenético comparativo entre dos variedades
1 Department of Botany, Jagannath University, Dhaka-1100, Bangladesh.
2 Cytogenetics Laboratory, Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh.
Karyotypes of two Colocasia oresbia botanical varieties from Bangladesh were analyzed and compared with orcein, chromomycin A3 (CMA) and 4´-6 diamidino-2-phenylindole (DAPI). Both varieties had 2n=2x=26 chromosomes (karyotypic formula: 20m+6sm) and a pair of satellites each. Total chromosome length was 144.18 ± 2.45 μm in C. oresbia var. oresbia and 133.02 ± 2.75 μm in C. oresbia var. stolonifera. The karyotype of Colocasia oresbia var. oresbia is 2A whereas that of C. oresbia var. stolonifera is 1A. Six CMA and four DAPI bands were observed in C. oresbia var. oresbia and eight CMA and six DAPI bands in C. oresbia var. stolonifera. However, in these two morphologically distinct C. oresbia varieties of two different ecological zones, the same somatic chromosome number, diversification in various karyotypic parameters and CMA/DAPI-banding patterns were observed. In addition to taxonomic characters, the studied karyotype features will contribute to the characterization of these two C. oresbia varieties and to establish a base for future research.
Key words: Chromosome banding; CMA; DAPI; Karyotype
Se analizaron y compararon los cariotipos de dos variedades botánicas de Colocasia oresbia de Bangladesh con orceína, chromomicina A3 (CMA) y 4-6 diamidino-2-phenilindol (DAPI). Ambas variedades presentaron 2n=2x=26 cromosomas (fórmula cariotípica: 20m+6sm) y un par de satélites cada una. La longitud total de cromosomas fue 144,18 ± 2,45 μm en C. oresbia var. oresbia y 133.02 ± 2.75 μm en C. oresbia var. stolonifera. El cariotipo de Colocasia oresbia var. oresbia es 2ª, y 1ª el de C. oresbia var. stolonifera. Se observaron seis bandas CMA y cuatro DAPI en C. oresbia var. oresbia y ocho bandas CMA y seis DAPI en C. oresbia var. stolonifera. Sin embargo, en estas dos variedades morfológicamente distintivas de C. oresbia de dos zonas ecológicas diferentes se observó el mismo número cromosómico somático, diversificación en varios parámetros cariotípicos y en patrones de bandeo CMA/DAPI. En adición a los caracteres taxonómicos, las características de los cariotipos estudiados contribuirán a la caracterización de estas dos variedades de C. oresbia y a establecer una base para futuras investigaciones.
Palabras clave: Bandeo cromosómico; CMA; DAPI; Cariotipo
ACKNOWLEDGEMENT
The authors are grateful to Dr. Hosene Ara, Ex- Director (In-charge), Bangladesh National Herbarium, Chiriakhana Road, Mirpur-1, Dhaka-1216, for providing the research materials and necessary information about Colocasia oresbia.
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Received: March 15, 2020; Accepted: June 18, 2020
This is an open-access article distributed under the terms of the Creative Commons Attribution License
