Resumen

LUCHETTI, C.G.; MOTTA, A.B.  y  LOMBARDO, D.M.. Antioxidant effect of metformin in a murine model of hyperandrogenized pregnancy. InVet [online]. 2019, vol.21, n.1, pp.01-17. ISSN 1668-3498.

Hyperandrogenization is one of the main clinical features of the polycystic ovarian syndrome (PCOS). Metformin (M) is a non-hormonal treatment used in PCOS even during pregnancy. The objective was to study the effects of M on the redox balance and the nitric oxide (NO) system in a murine model of early hyperandrogenized pregnancy. Early pregnant Balb/ c mice hyperandrogenized by dehydroepiandrosterone (DHEA) and treated orally with M were used. Redox and NO system parameters were determined at the implantation sites. DHEA increased oxidative stress: lipid peroxidation (TBA-RS, p <0.01) and glutathione (Tietze; p <0.01). With DHEA + M, TBA-RS was similar to the control and glutathione was similar to the DHEA group. The enzymes superoxide dismutase and catalase did not show differences. DHEA caused an increase in the expression of inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) (immunohistochemistry) and in NO (Griess; p <0.001 in all cases). With DHEA+M they were similar to the control. We conclude that M avoids the effects of DHEA on oxidative stress and the NO system in the implantation sites of hyperandrogenized early pregnant Balb/c mice.

Palabras clave : DHEA; Oxidative stress; NO; Polycystic ovary syndrome; Implantation sites.