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Latin American applied research
versão impressa ISSN 0327-0793versão On-line ISSN 1851-8796
Resumo
HERNANDEZ-LOPEZ, J.L.. High-sensitive bioactivity assay for hybrid nanostructured materials with plasmonic properties. Lat. Am. appl. res. [online]. 2014, vol.44, n.2, pp.119-121. ISSN 0327-0793.
The binding of biotin to gold nanorods (Au-NRs) conjugated to neutravidin is accompanied by an increase in the fluorescence intensity of protein's tryptophan residues. Localized surface plasmon resonance (LSPR), combined with the known affinity of streptavidin for biotin, led to the development of a high-sensitive enzyme activity assay.
Palavras-chave : Au-NRs/Neutravidin; LSPR; Enzyme Activity; In-vivo Imaging; Biological Sensing..