Medicina (Buenos Aires)
versión ISSN 0025-7680
NOTARIO, Rodolfo et al. Quinolone resistant Campylobacter jejuni strains isolated from humans and from poultry. Medicina (B. Aires) [online]. 2011, vol.71, n.4, pp. 331-335. ISSN 0025-7680.
Eight quinolone resistant Campylobacter jejuni strains isolated from humans with diarrheal disease were compared with 23 isolates from chicken and from laying hens. Samples were cultured on selective agar in microaerophilia, identified by conventional tests, and conserved in 17% glycerol at -70 °C. Clones were determined by RAPD-PCR employing the 1254 primer (Stern NJ). Five patterns were obtained. Patterns I, II, and V were found in both poultry and human isolates. Pattern I was obtained from poultry in a domestic henhouse. Pattern III was only obtained from humans whereas pattern IV was only obtained from poultry. A 95.3% of clones were found in both, humans and poultry. According to these results colonization by quinolone resistant strains could be the origin of this human infection, acquired by ingestion.
Palabras llave : Campylobacter jejuni; Poultry; Laying hens; Quinolones resistance; RAPD-PCR.