Acta bioquímica clínica latinoamericana
versión On-line ISSN 1851-6114
MORETTI, Edgardo et al. Detection of antibodies to Trypanosoma cruzi andToxoplasma gondii in oral mucosal transudate. Acta bioquím. clín. latinoam. [online]. 2004, vol.38, n.2, pp. 159-163. ISSN 1851-6114.
Oral mucosal transudate (OMT) is a biological fluid that can be obtained by an absorbent pad placed between lower cheek and gum, and contains20% IgG, 40% IgA and 10% IgM comparing with serum. The aim of this work was to analyse the performance of OMT as biological material to detect antibodies in Chagas' disease and Toxoplasmosis. Sera of ambulatory patients, pregnant women and healthyvolunteers were tested for Chagas and Toxoplasmosis employingImmunofluorescence, ELISA andHemagglutination.OMT of the same patients were assayed by ELISA, and the results compared to determinate sensibility, specificity and predictive value. In Chagas serology, three different commercial kits were assayed. The sensibility ranged from 91 to 100%,specificitybetween 90 and 100%. The predictive values oscillate between 95% and 99%. The studies in Toxoplasmosis did not shown false positive results. The sensibility was 95%, specificity 100% and the predictive values between 98% and100%. Sera from neonates born from Toxoplasmosis infected mothers were also studied, and the results were in agreement with reference tests. These results suggest that OMT could be a suitable alternative biological fluid in immunoepidemio-logical surveys, and also as screening test in the diagnosis and prevention of materno-fetal transmission of infectious diseases.
Palabras clave : Oral mucosal trasudate.; Chagas; Toxoplasmosis; Immunodiagnosis; Seroepidemiological surveys.