SciELO - Scientific Electronic Library Online

 
vol.38 número2Resistencia de Streptococcus pyogenes a los antibióticos: Experiencia de once años en un hospital pediátrico de Buenos AiresEl dilema de la obesidad en ambos sexos índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Articulo

Indicadores

  • No hay articulos citadosCitado por SciELO

Links relacionados

  • En proceso de indezaciónCitado por Google
  • No hay articulos similaresSimilares en SciELO
  • En proceso de indezaciónSimilares en Google

Bookmark


Acta bioquímica clínica latinoamericana

versión On-line ISSN 1851-6114

Resumen

MORETTI, Edgardo et al. Detection of antibodies to Trypanosoma cruzi andToxoplasma gondii in oral mucosal transudate. Acta bioquím. clín. latinoam. [online]. 2004, vol.38, n.2, pp. 159-163. ISSN 1851-6114.

Oral mucosal transudate (OMT) is a biological fluid that can be obtained by an absorbent pad placed between lower cheek and gum, and contains20% IgG, 40% IgA and 10% IgM comparing with serum. The aim of this work was to analyse the performance of OMT as biological material to detect antibodies in Chagas' disease and Toxoplasmosis. Sera of ambulatory patients, pregnant women and healthyvolunteers were tested for Chagas and Toxoplasmosis employingImmunofluorescence, ELISA andHemagglutination.OMT of the same patients were assayed by ELISA, and the results compared to determinate sensibility, specificity and predictive value. In Chagas serology, three different commercial kits were assayed. The sensibility ranged from 91 to 100%,specificitybetween 90 and 100%. The predictive values oscillate between 95% and 99%. The studies in Toxoplasmosis did not shown false positive results. The sensibility was 95%, specificity 100% and the predictive values between 98% and100%. Sera from neonates born from Toxoplasmosis infected mothers were also studied, and the results were in agreement with reference tests. These results suggest that OMT could be a suitable alternative biological fluid in immunoepidemio-logical surveys, and also as screening test in the diagnosis and prevention of materno-fetal transmission of infectious diseases.

Palabras llave : Oral mucosal trasudate.; Chagas; Toxoplasmosis; Immunodiagnosis; Seroepidemiological surveys.

        · resumen en Español     · texto en Español     · pdf en Español