Acta bioquímica clínica latinoamericana
versión On-line ISSN 1851-6114
MOLINA, Nora Beatriz; MINVIELLE, Marta Cecilia y BASUALDO, Juan Angel. RK13 Cells: Influence of fetal bovine serum concentration on DOubling time. Acta bioquím. clín. latinoam. [online]. 2004, vol.38, n.4, pp. 477-480. ISSN 1851-6114.
The aim of this study was to analyze the Doubling Time (DT) and the Lag phase of a cell line with two concentrations of fetal bovine serum (FBS) and two initial inocula. Four growth curves of RK13 cells in Minimal Essential Medium (MEM) supplemented with glutamine, penicillin, streptomycin and 5% and 7% non-inactivated FBS were performed. The initial inocula were 3 x 104 and 6.8 x 104 viable cells/mL. DT was 24 h for the culture with 7% FBS with the two inocula and 16.36 h for the culture with 5% FBS with the greatest inoculum. The culture with 5% FBS and 3 x 104 viable cells/mL had limited growth. There was significant difference in DT (p = 0.0055) when the inoculum was 6.8 x 104 viable cells/mL with the two concentrations of FBS. There was no significant difference with the 7% FBS and the two inocula (3 x 104 and 6.8 x 104 viable cells/mL).
Palabras clave : doubling time; RK13; cell culture; fetal bovine serum; cell inoculum.