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Acta bioquímica clínica latinoamericana

Print version ISSN 0325-2957On-line version ISSN 1851-6114


BOVONE, Nora Silvia; FUENTE, María Cristina; EPOSTO, Mario Omar  and  LONDERO, Paula. Anti-nucleus antibodies detection by line immunoassay. Correlation with indirect immunofluorescence. Acta bioquím. clín. latinoam. [online]. 2005, vol.39, n.4, pp.423-428. ISSN 0325-2957.

The most used screening test to detect autoantibodies in systemic diseases is indirect immunofluorescence (IIF) with Hep-2 cells. However, additional methods, such as double diffusion, immunoblotting, ELISA or line immunoassay (LIA), are used to identify specific antibodies profiles. The aim of this study was: a) To asses the probability of fine detection by a commercial LIA on serum samples that resulted positive by IIF screening and b) To asses the correlation between the patterns and titles observed. A hundred and sixty-one serum samples positive by IIF that had also been processed by LIA were selected. Hep-2 slides of Kallestad and commercial kit Innolia-ANA of Innogenetics were used. Sixty samples were also studied for anti-dsDNA by IIF with Crithidia Lucilliae slides of Kallestad. Sixty per cent of the samples with homogeneus pattern were positive by LIA, with a prevalence of anti-histones and anti-dsDNA. The finding of antibodies anti-ENA in this group was important. In the speckle sample, 73% were positive. In detecting anti-ENA, speckle sample was more forceful than homogeneous pattern which showed little information about a specific antibodies profile. Anticentromere pattern did not need confirmation. The probability of finding fine reactivity by LIA is increased with the rise in title, but its search for low titles is still justified.

Keywords : autoantibodies; indirect immunofluorescence; immunoassay; nonparametric statistics.

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