Acta bioquímica clínica latinoamericana
versión On-line ISSN 1851-6114
DUBINSKY, Valeria; GISELA, Junovich y GUTIERREZ, Gabriela. Innate Immune Response between environmental factors and abortion: lipopolysaccharide tolerization model during gestation. Acta bioquím. clín. latinoam. [online]. 2006, vol.40, n.1, pp. 71-76. ISSN 1851-6114.
The exacerbated local inflammatory response that triggers immunological abortion on CBA/j x DBA/2 murine model depends on environmental factors such as LPS. Since deficient Tlr4 mice are resistant to LPS-induced abortion, the effect of LPS tolerization on the prevention of fetal losses, as well as on the synthesis of placental cytokines on CBA/j x DBA/2 model has been studied. Low doses of LPS were inoculated during pre and post-implantatory stages on CBA/j pregnant mice. In order to investigate tolerization effect, abortogenic doses of LPS were added to placental and splenocyte cultures. Results obtained with post-implantatory inoculation of 1µg/day LPS showed a decrease in the abortion rate as well as an IL-6 hyporesponsive to LPS booster, both in placenta and splenocyte cultures. However, stimulation with abortogenic dose of LPS modified TNF-a levels by an increase in placenta but a decrease in splenocyte culture supernatants. Considering these results, it can be concluded that LPS tolerance induction depends on the organ as well as on the cytokine studied.
Palabras clave : abortion; innate immune response; lipopolysaccharide; tolerization; cytokines.