SciELO - Scientific Electronic Library Online

vol.40 número1 índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados




  • No hay articulos citadosCitado por SciELO

Links relacionados


Acta bioquímica clínica latinoamericana

versión impresa ISSN 0325-2957versión On-line ISSN 1851-6114


PERSUHN, Darlene Camati et al. Optimization of Methylenetetrahydrofolate Reductase C677T polimorphism detection. Acta bioquím. clín. latinoam. [online]. 2006, vol.40, n.1, pp.97-101. ISSN 0325-2957.

A common mutation C677T in methylenetetrahydrofolate reductase (MTHFR) gene, involved in the metabolism of homocysteine, has been suggested to play a role in increasing cardiovascular disease risk. In order to facilitate the obtainment of population data, the objective of this work was improve its PCR detection. Leucocytes DNA purification alternative protocols were tested and a new low cost method of NaCl precipitation was developed. The composition of PCR mixture was also tested and it defined that the concentration of deoxynucleotides, primers and Taq DNA polimerase could be reduced in a half. The final volume of PCR mixture was reduced to 15 µL, enabling the utilization of the same tube to amplification and HinfI digestion processes, avoiding extra steps. The significance of cardiovascular disease in populational data and the possibility of involvement of this mutation as risk factor of precocious ACD (arterial cardiovascular disease) justify this work.

Palabras clave : methylenetetrahydrofolate reductase; C677T; PCR.

        · resumen en Portugués     · texto en Portugués     · Portugués ( pdf )


Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons