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Acta bioquímica clínica latinoamericana

Print version ISSN 0325-2957On-line version ISSN 1851-6114


PONCE DE LEON, Patricia; FORESTO, Patricia  and  VALVERDE, Juana. Ascaris lumbricoides: P system epithopes by haemogglutination kinetics method. Acta bioquím. clín. latinoam. [online]. 2007, vol.41, n.1, pp.77-81. ISSN 0325-2957.

Summary Previous experiences have demonstrated P1 epithopes in Ascaris lumbricoides by inhibition agglutination. Haemogglutination kinetics applies the relative optical extinction produced on a light beam transmitted through a suspension of small particles, giving a parametric estimate of the haemagglutination rate.The aim was to use this method for the demonstration of P System epithopes presence in A. lumbricoides extracts. inhibition agglutination test enabled the selection of 10 extracts: 3 with and 7 without P1 epithopes. Kinetics of anti- P1 - P1 erythrocyte control reaction and kinectics of the same reaction with a previous contact between antibody and extract were registered. 3 EOR % values (relative optical extinction variation) for both reactions were calculated and compared between themselves. The results proved P1 epithopes presence in 3 extracts, which had not been detected by inhibition agglutination. The results coincided for the remaining extracts by both methods. Haemogglutination kinetics is simple and fast and it is easily adapted for experiences in which the investigator needs to assess the antibody-antigen reaction by haemogglutination.

Keywords : haemogglutination kinetics; P1 epithopes; Ascaris lumbricoides.

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