Acta bioquímica clínica latinoamericana
versión On-line ISSN 1851-6114
PONCE DE LEON, Patricia; FORESTO, Patricia y VALVERDE, Juana. Ascaris lumbricoides: Inhibitory capacity in relation to P1 antigen. Acta bioquím. clín. latinoam. [online]. 2007, vol.41, n.2, pp. 225-228. ISSN 1851-6114.
P Blood Group has a unique antigen: P1. It was identified in various parasite species. The aim of this work was to determine A. lumbricoides´s Inhibitory Capacity in relation to P1 antigen. The work was based on 11 parasite extracts (AE). The semiquantitative inhibition agglutination technique was used to assess the extracts´ Inhibitory Capacity in relation to P1 antigen. Two series of anti- P1 monoclonal antibody dilutions were prepared. PBS was added to the first series and an equal volume of AE to the second. Suspensions of P1 red cells were used as a revealing system. Monoclonal antibody titres were determined in both series. The agglutination was considered significative when there was a difference of two or more dilutions between the titres. Eight of the 11 extracts studied presented P1 epithopes. Inhibitory power of these 8 extracts was calculated and it was related with their protein concentrations to compare the Inhibitory Capacity among the extracts. The technique used made it possible to prove P System epithopes presence and to compare the extracts´ Inhibitory Capacity in relation to P1 epithopes.
Palabras clave : Ascaris lumbricoides; inhibitory capacity; P1 antigen.