Abstract

DETARSIO, Germán Alejandro et al. Lupus Anticoagulant: Sensitivity of 19 commercial activated partial thromboplastin time reagents. Acta bioquím. clín. latinoam. [online]. 2007, vol.41, n.4, pp.533-539. ISSN 0325-2957.

Lupus anticoagulants (LA) are immunoglobulins which interfere in in vitro phospholipid-dependent coagulation tests. Various methods have been proposed; however, activated partial thromboplastin time (APTT) is the most used screening test for lupus anticoagulant. Previous studies have shown that sensitivity to the lupus anticoagulant defect varies considerably with different APTT reagents. In view of the undoubted clinical importance of lupus anticoagulants, the sensitivity of 19 commercial APTT reagents has been evaluated. The study raises several important conclusions: No difference was found in sensitivity associated with a narrower distribution of liposomes' diameter, considering the latter as a marker of uniformity in phospholipid distribution. The source of the platelet substitute and the nature of the contact phase activator are unlikely to determine such varied sensitivity. Finally, a significant negative correlation between APTT sensitivity and total phospholipid concentration was found. The lower the phospholipid concentration, the higher the APTT sensitivity to AL.

Keywords : Lupus anticoagulant; Activated partial thromboplastin time; Antiphospholipid syndrome.

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