Resumen

DUBOSCQ, Cristina  y  KORDICH, Lucía. Influence of preanalytical variables in S protein determination. Acta bioquím. clín. latinoam. [online]. 2008, vol.42, n.1, pp.89-93. ISSN 0325-2957.

Protein S has an essential anticoagulant function acting as activated Protein C cofactor and forming an equimolecular complex with it. In the presence of calcium this complex regulates the coagulation process inactivating thrombin activated factors V and VIII by proteolysis. In plasma there are two different forms: a) free Protein S which acts as the cofactor of activated protein C (representing about 40% of total Protein S) and b) C4-BP(C4 binding protein) bound protein S which exhibits no activity as cofactor of activated Protein C (representing about 60% of total PS). The objetive was to compare the PS dosage determination by two methods: immunoturbidimetric and clotting, and to investigate how pre-analytical variables affect the results. The following results were obtained: Clotting method: CV intra assay: (n=20): 4%, CV interassay (n=20, 3 days): 3.4%; immunoturbidimetric method CV intra assay (n=20): 3.7%: CV inter assay (n=20.3 days): 4.5%. There is a good correlation (R2 = 0.94) between both methods; when the clotting method is calibrated in batch with the samples. There is significant difference between fresh and frozen ( -20 °C and -80 °C) samples when the latter have been desfrozen only once.

Palabras clave : Protein S; Free protein S; Prenalytical variables.

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