Acta bioquímica clínica latinoamericana
versión On-line ISSN 1851-6114
PONCE DE LEON, Patricia; FORESTO, Patricia y VALVERDE, Juana. Identification of ABO Blood Group like epitopes in vitro maintained Ascaris lumbricoides larvae. Acta bioquím. clín. latinoam. [online]. 2009, vol.43, n.1, pp. 21-26. ISSN 1851-6114.
Previous experiences have demonstrated the same ABO System epitopes in A. lumbricoides extracts and in their hosts. The aims of this work were to study ABO antigenic determiner absorption by in vitro culture of the helminth's larvae and to investigate ABO-Blood-Group-like substances in larvae's excretion/ secretion products. Larvae were obtained by hatching of A. lumbricoides eggs. The larvae were collected in 5 tubes with RPMI 1640 medium by the Baermann method. A, B and O erythrocytes were added in 3 of the tubes. The two remaining tubes were used as absorption Negative Control and to investigate larvae's excretion/secretion products. The Semiquantitative Inhibition Agglutination Test was applied by using monoclonal antibodies. The results showed that the larvae may adsorb A, B and H epitopes from the erythrocytes that were added to the culture medium. A and B-Blood-Group-like substances were identified in the excretion/secretion products. The experience can contribute to understand the host's escape mechanism used by A. lumbricoides.
Palabras clave : ABO epitopes; Culture; Larvae; Ascaris lumbricoides.