SciELO - Scientific Electronic Library Online

 
vol.44 número2Carotenoides en quimioprevención: LicopenoBacteriemias causadas por Acinetobacter spp. y resistencia a carbapenemes índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

  • No hay articulos citadosCitado por SciELO

Links relacionados

Compartir


Acta bioquímica clínica latinoamericana

versión impresa ISSN 0325-2957

Resumen

BLANCO, María Alejandra; MONACO, María Belén  y  LOPARDO, Horacio Angel. Evaluation of an automated system for identifying enterococcal species. Acta bioquím. clín. latinoam. [online]. 2010, vol.44, n.2, pp.239-242. ISSN 0325-2957.

It has been demonstrated that the Vitek system is almost equivalent to conventional biochemical tests for identifying clinical isolates of Enterococcus spp. to the species level. However its performance is uncertain when less frequently found species are being identified. In the present study the ability of a commercial system (Vitek system) to identify enterococcal species was evaluated and compared to the conventional scheme. A total of 100 isolates of Enterococcus were tested: Enterococcus faecalis (25), Enterococcus faecium (25), Enterococcus gallinarum (21), Enterococcus casseliflavus (2), Enterococcus raffinosus (16), Enterococcus durans (4), Enterococcus hirae (2), Enterococcus mundtii (1) and Enterococcus avium (4). It is considered that one isolate was correctly identified when the percentage of the first choice was over 90% by the Vitek system. When this percentage was lower: (a) database test, (b) final point test, and/or (c) additional tests (alpha-methyl-D-glucopyranoside and motility) were analyzed. Of the 100 tested isolates, 56 were correctly identified using no additional tests. Using a corrective action a, b and c, the percentage increased to 85%. In conclusion, the use of Vitek version VTK-R 9.01 is not a reliable method for identification of enterococci unless the method described in this study be applied.

Palabras clave : Vitek; Enterococcus; Identification.

        · resumen en Español     · texto en Español     · Español ( pdf )

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons