Acta bioquímica clínica latinoamericana
versión impresa ISSN 0325-2957
PONCE DE LEON, Patricia; BIONDI, Claudia y VALVERDE, Juana. Effect of Ascaris lumbricoides on the erythrocyte superficial charge using Polybrene Method. Acta bioquím. clín. latinoam. [online]. 2010, vol.44, n.4, pp. 689-696. ISSN 0325-2957.
Sialic acids (SA) contained in glycoproteins and glycolipids participate in various biological functions; besides, its presence on the erythrocyte surface has both hemodynamical and hemorheological importance. SA are thought to intervene in the parasite-host interaction. The aim of this work was to study the effect of Ascaris lumbricoides on the erythrocyte superficial charge by using Polybrene Method. Work was carried out on 51 adult parasite extracts ([AE]) and 4 (larvae) larvae concentrates ([ALLC]: 2000 / 1000 / 500 / 250 larvae/ mL). The method was applied to non-treated (Control) and treated red cells with [AE] / all 4 [ALLC] simultaneously. Larvae´ treatment was conducted over 18 experiences. Erythrocytes treated with 37. 25% of the [AE] had lower aggregation than the Control. The statistical analysis showed that this effect was not related to the protein concentration of [AE]. The aggregate decrease in the treatment with [ALLC] was dependent on larvae concentration and it was related to larvae concentrations = 500 larvae/ mL. It was observed that the [AE]/ [ALLC] fixation to the erythrocyte was not strong. The results have shown that A. lumbricoides sequestrates SA erythrocyte. This capture could affect the pathology and/or participate in the escape of the host's immune response.
Palabras clave : Ascaris lumbricoides; Erythrocyte charge; Polybrene.