Acta bioquímica clínica latinoamericana
versión impresa ISSN 0325-2957
PONCE DE LEON, Patricia; DI VITA, Santiago; BIONDI, Claudia y VALVERDE, Juana. Absortion of ABH soluble antigens by Ascaris lumbricoides larvae in vitro. Acta bioquím. clín. latinoam. [online]. 2011, vol.45, n.1, pp. 125-131. ISSN 0325-2957.
Previous experiences have identified ABH antigens in A. lumbricoides's adult specimens. The parasites only showed epithopes expressed in their respective hosts. It was demonstrated that epithopes are acquired by absorption by means of the culture of larvae with erythrocytes. As the adult parasite lives in the small intestine, it could be present there because the fourth stage larva retains the epithopes absorbed in the migration and keeps them during the maturation process to adult, or the adult specimen would acquire them from intestinal secretions. Larvae were obtained by hatching of eggs and they were collected and cultured in RPMI medium. The aim of this work was to study the ABH soluble epithopes by larvae in culture. B soluble antigen was added into three tubes, which were incubated for 2, 3 and 6 days, and A soluble antigen was poured into two tubes, both cultured for 6 days. Each culture time had its absorption Negative Control where ABH like antigen excretion/secretion products (ES) were investigated. The Semiquantitative Inhibition Agglutination Test showed B epithopes in the larvae cultured for 3 days and A and B epithopes in the larvae incubated for 6 days. Similar A and B ES products were only evidenced in the Control of 6 days. The results conclude that A. lumbricoides may absorb ABH antigenic determiners from soluble antigens.
Palabras clave : Absorption; ABH soluble antigens; Ascaris lumbricoides.