SciELO - Scientific Electronic Library Online

 
vol.45 número3Qualidade no laboratorio de urgências: determinaçâo do estado ácido-baseMetodologia UV para a determinação dos antichagásicos Nifurtimox e Benznidazol em sangue índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

  • Não possue artigos citadosCitado por SciELO

Links relacionados

Compartilhar


Acta bioquímica clínica latinoamericana

versão impressa ISSN 0325-2957

Resumo

PONCE DE LEON, Patricia; DI VITA, Santiago; BIONDI, Claudia  e  VALVERDE, Juana. Sialic acid capture by Ascaris lumbricoides larvae during in vivo incubation. Acta bioquím. clín. latinoam. [online]. 2011, vol.45, n.3, pp.455-461. ISSN 0325-2957.

Erythrocyte aggregation affects microcirculation and its study is important in vascular diseases. It was communicated that Ascaris lumbricoides can capture erythrocyte sialic acid (SA) and alter the anionic charge on the red cell. The aim of this work was to study SA capture by A. lumbricoides larvae incubated in vivo with erythrocytes. Work was performed with concentrated larvae ([ALLC]) incubated in 4 tubes with phosphate buffer and antibiotics. Group O erythrocytes were added in two Tubes. The remaining were Controls. They were incubated at 37 °C (5% CO2) for 24 and 28 hours. The larvae were separated, collected, concentrated and counted microscopically (larvae/mL: [ALLC]1:1500-1700; [ALLC]3: 1600-1800; [ALLC]2 and 4: 200- 400). Aggregation Inhibition by Polybrene (AIP) and Blue Alcian (BA) techniques were used. AIP showed a significant difference between Polybrene Titers diluted with physiological solution and with [ALLC]1 and 2, which were incubated with erythrocytes 24 and 48 hours respectively. There was no change in the Title of the corresponding Controls. BA performed in [ALLC]1 and [ALLC]3 determined CapSAC = 6.65% ± 0.36 and SAC[CLAL]3 %= 0.67% ± 0.36. The experience showed SA capture by larvae incubated in vivo with erythrocytes and it suggested that they have no intrinsic SA. SA kidnapping during the larval migration could be important in parasite-host interaction.

Palavras-chave : Capture; Sialic acid; Larvae; Ascaris lumbricoides.

        · resumo em Português | Espanhol     · texto em Espanhol     · Espanhol ( pdf )

 

Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons