SciELO - Scientific Electronic Library Online

vol.46 issue1Valuation of platelet índices in thrombocytopeniaLiver function tests in patients with acute viral infection author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Acta bioquímica clínica latinoamericana

Print version ISSN 0325-2957


QUERALES, Marvin et al. Measurement of cholesterol low-density lipoprotein using three methodologies. Acta bioquím. clín. latinoam. [online]. 2012, vol.46, n.1, pp. 31-38. ISSN 0325-2957.

Considering that there is still no standard methodology for routine determination of low density lipoprotein (LDL-c) it was decided to evaluate their analytical determination using three techniques: homogeneous enzymatic determination, polyvinyl sulphate precipitation and Friedewald formula. Ninety-eight serum samples were processed; triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL-c) and LDL-c were determined. Mean total cholesterol was 194.46 ± 43.54 mg/dL, HDL-C was 51.12 ± 12.36 mg/dL and TG was 132.88 ± 76.93 mg/dL. Although regression analysis showed a good correlation between LDL-c, the results showed a statistically significative difference in them when TG levels exceeded 200 mg/dL. It was mainly observed in the precipitation method and the Friedewald formula, the latter values being significantly lower (LDL-C by precipitation: 141.3 ± 26.2 mg/dL, LDL-C by the Friedewald formula: 110, 1 ± 35.4 mg/dL). Moreover, this difference affected the proportion of individuals classified according to their coronary risk. It is necessary to compare the techniques applied in this study with beta quantification to assess which has a higher level of accuracy.

Keywords : Low density lipoproteins; Homogeneous enzymatic method; Precipitation method; Friedewald formula.

        · abstract in Spanish | Portuguese     · text in Spanish     · Spanish ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License