Acta bioquímica clínica latinoamericana
Print version ISSN 0325-2957
VAUSTAT, Claudia Daniela and BAVA, Amadeo Javier. Microscopio examination in fresh mount preparation for the diagnosis of pulmonary pneumocystosis. Acta bioquím. clín. latinoam. [online]. 2012, vol.46, n.1, pp. 69-72. ISSN 0325-2957.
To evaluate the usefulness of fresh microscopy for the diagnosis of pulmonary pneumocystosis (PCP), direct immunofluorescence (DIF) with monoclonal antibodies technique was applied to 50 respiratory secretions obtained by bronchoalveolar lavage and consecutively processed in the Laboratory of Parasitology from January 19, 2011 to February 25, 2011. The samples belonged to AIDS patients with presumptive diagnosis of PCP, and all of them were negative for the search of foamy exudates by wet mountmicroscopy. No positive results were obtained for Pneumocystis jiroveci with the DIF. According to the results obtained, it was concluded that fresh microscopy remains being a rapid, economic, simple and accurate method for the diagnosis of PCP in AIDS patients assisted in different Wards of the Muñiz Hospital. As in a previous study, performed in a similar cohort of patients, fresh microscopy revealed a sensitivity similar to that of DIF when applied to the diagnosis of PCP.
Keywords : Fresh microscopy; Direct immunofluorescence; Pneumocystosis; Pneumocystis jiroveci.