Acta bioquímica clínica latinoamericana
versão ISSN 0325-2957
POMILIO, Alicia Beatriz et al. Heterologous neutralization and interaction of toxic amanitins with Bothrops and Crotalus venoms, and honey bee venom. Acta bioquím. clín. latinoam. [online]. 2012, vol.46, n.2, pp. 171-182. ISSN 0325-2957.
In the present work, the interaction of the amanitins of Amanita phalloides (Basidiomycetes) with the venoms of Bothrops neuwiedi diporus ("small yarará snake"), B. alternatus ("big yarará"), Crotalus durissus terrificus ("rattlesnake"), and honey bee Apis mellifera was examined. Ouchterlony, immunotransfer, rocket-electrophoresis, and polyacrylamide gel electrophoresis techniques were applied to anti-venoms and anti-toxins obtained by immunization in horses and/or in rabbits. Snake antisera and amanitins cross-reacted as well as bee venom and amanitins. When venoms of Bothrops neuwiedii diporus and Crotalus durissus terrificus were preincubated with amanitins and analysed by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE), some protein bands disappeared and others were significantly reduced. These results reveal for the first time the interaction and degradation of proteins in snake venoms by amanitins. Moreover, the modification of the human blood clotting time due to snake venoms was corrected by the Amanita cyclopeptides. These results are also reported for the first time in this work. The occurrence of toxic polypeptides in the snake and bee venoms as well as in A. phalloides, and the cross-reactivity demostrated herein, suggest the occurrence of epitopes common to all of them. Taking into account these reactions,the use of heterologous anti-venoms seems to be of value in envenomation treatment.
Palavras-chave : Amanita phalloides; Apis mellifera mellifera; Bothrops neuwiedi diporus; Bothrops alternatus; Crotalus durissus terrificus; Cross-reactivity; Interaction; Sodium dodecylsulfate-polyacrylamide gel electrophoresis; Human clotting time.