Acta bioquímica clínica latinoamericana
versão impressa ISSN 0325-2957
VELIZ, María Angélica et al. Iron liver quantification using electrothermal atomic absorption spectrometry. Acta bioquím. clín. latinoam. [online]. 2012, vol.46, n.2, pp. 221-228. ISSN 0325-2957.
The iron (Fe) deposit was studied in the liver tissue of healthy and chronic liver disease subjects, all of them adults aged 46 to 70. The iron quantification was made by electrothermal atomic absorption spectrometry (ET AAS). Biopsy liver samples were weighed, measured, and put under acid digestion. Simultaneously a test was performed to analyze recovery in both strong samples and bound reagents with the addition of a standard of iron. Quantification was carried out using a calibration curve (from 10 to 50 mg/L): Peak areas of 248.3 nm were measured through a hollow cathode lamp. The results of the samples for healthy individuals were less than 1,000 µg/g of dry tissue, while the range in subjects with chronic liver disease was higher, (range from 800 to 7,835 µg/g dry tissue). There is a direct relationship between concentrations of iron in liver tissue and the range of the metal deposit for which reason development of the ET AAS methodology made it possible to quantify the iron overload and estimate the values associated to the different hepatopathies.
Palavras-chave : Iron; Iron overload; Human liver; Atomic absorption spectrometry.