Acta bioquímica clínica latinoamericana
versão ISSN 0325-2957
PONCE DE LEON, Patricia et al. Ascaris lumbricoides action on the erythrocyte and desyalated erythrocyte anionic charge. Acta bioquím. clín. latinoam. [online]. 2012, vol.46, n.2, pp. 247-256. ISSN 0325-2957.
The aim of this work was to study the A. lumbricoides' effect on the anionic charge of erythrocytes and desyalated erythrocytes. Work was carried out on 30 extracts ([AE]) and 2 larval concentrates ([ALLC] 1: 500-600; ([ALLC] 2: 200-300 larvae/ mL) of the parasite and Group O erythrocytes in saline (RC) and enzymatic medium (RCb). The red cell sediment was incubated with an equal volume of parasite and the Control (RCc) with saline solution, at 37C. Polybrene and Alcian Blue Methods were applied and Aggregation Titulation from Polybrene was implemented. The coefficients for each method were defined,which were analyzed statistically. The results showed that a longer treatment and also at higher larval concentration,increased the capture of sialic acid (AS),while decreasing the RC and RCb aggregation. The effect was greater on the incubation of the parasite with RCb in relation to RC. The enzymatic treatment reduced similarly the percentage charge in the globules incubated with both [ALLC] (16% -17% of initial) and the variation of RC and RCb charge due to both was about 10%,indicating that the charge loss caused by [ALLC]1 was significant with regard to [ALLC]2. We concluded that A. lumbricoides can capture erythroctyte SA. This phenomenon would contribute to explain the anaemia and thrombi produced in ascariosis,noting that in patients with AS deficit pathologies,these complications would be more relevant.
Palavras-chave : Ascaris lumbricoides; Erythrocyte charge.