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Acta bioquímica clínica latinoamericana

Print version ISSN 0325-2957


BOVONE, Nora Silvia  and  GASTIAZORO, Ana María. Analysis of agreement between the dosage of immunoglobulins and gamma fraction of serum protein electrophoresis. Acta bioquím. clín. latinoam. [online]. 2013, vol.47, n.3. ISSN 0325-2957.

Two criteria are proposed to evaluate the agreement between densitometry and nephelometry of serum immunoglobulin level, based on own patient population and its possible application in a self-validation algorithm. A difference GAMgamma = Sum Igs -gamma variable was constructed; analysis was performed according to the Bland and Altman method. CI 95% was estimated using as reference a cohort of 497 patients attending Posadas Hospital's laboratory in October and November 2011 who had a normal dosage of G, A and M immunoglobulins for an adult population. These results were compared with two populations: a) 977 patients attending the hospital in the same period and b) 111 patients with monoclonal component (MC). In the reference population, the CI 95% Difference (mg/dL) was 115-653 and the limits were ± 21 mg/dL. Using Reference Values criteria followed by Difference, the following results were obtained: in the patient population, 470/977 (48.1%) passed both alarms and will be validated. In the MC population, 75.6% gave at least one alarm. However, although this may be considered acceptable sensitivity, there were 27 MC who passed both alarms. It can be concluded that after a visual inspection, almost 50% of the results will be self-validated.

Keywords : densitometry; nephelometry; immunoglobulins; agreement; Bland and Altman.

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