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Acta bioquímica clínica latinoamericana

Print version ISSN 0325-2957


ZOTTA, Claudio Marcelo et al. Genetic diversity of Escherichia coli 0145:NM/H27 strains isolated in Buenos Aires province. Acta bioquím. clín. latinoam. [online]. 2014, vol.48, n.1. ISSN 0325-2957.

SHIGA TOXIN-PRODUCING Escherichia coli (STEC), especially the serotype 0157-.H7, is associated with the occurrence of sporadic cases and outbreaks of bloody diarrhea (DS) and hemolytic uremic syndrome (HUS). Other STEC serotypes such as 026:H11, 0103:H2, 0111:NM, 0113:H21, and 0145:NM can also cause severe human illness. In Argentina 0157:H7 is prevalent, followed in frequency by serotype 0145:NM. The aim of this study was to determine the genetic diversity and clonal relationship of E. coM 0145:NM/ H27 strains isolated in different locations of the Buenos Aires province, in the period 2004-2009. A total of 17 strains isolated from HUS (9), DS (5) and asymptomatic contacts (3) were characterizea by POR to detect stXj, stx2, rfbO157, eae and ehxA genes, and pulsed-field gel electrophoresis (Xbal-PFGE) to establish the genetic diversity and the clonal relationship. All isolates were characterized as stx2/eae/ehxA. By Xba\-PFGE, 14 different patterns were established, with a single cluster identified. A regional database of E. coii 0145:NM/H27 was created, which will monitor the \-PFGE profiles of the circulating strains in order to identify the probable occurrence of an outbreak in the community and report to the authorities to implement control measures.

Keywords : genetic diversity; clonal relationship; Escherichia coli O145.NM/H27; pulsed-field gei electrophoresis.

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