Abstract

PONCE DE LEON, Patricia; LOPEZ MURUA, Gabriel  and  RACCA, Liliana. Study of Trichinella spiralis effect on desialylation by Polybrene Method. Acta bioquím. clín. latinoam. [online]. 2015, vol.49, n.3, pp.347-352. ISSN 0325-2957.

Previous experiences reported that erythrocytes (RBCs) incubated with newborn larvae (NL) and muscle larvae (ML) of T. spiralis present lower sialic acid than corresponding Control RBCs. The objective of this work was to study the effect of NL and ML on desialylation applying Polybrene Method. Work was performed with NL and ML concentrates, which were incubated with an equal volume of O Group RBCs in saline and enzymatic mediums, for 120 minutes at 30-minute intervals. Control RBCs were incubated with an equal volume of saline solution. Polybrene Method was applied and the value of CexpCASP coefficient was calculated. To analyze the effect of the parasite, incubation medium and treatment time on the CexpCASP values, a multifactorial ANOVA was used. The results showed that enzymatic medium and increasing the incubation time produce the greatest decrease in globular charge, which is reflected in lower coefficient values. No significant differences were observed with the larval stage used. The experience would suggest that during their migration through the bloodstream, the NL could capture erythrocyte sialic acid and alter the hemorrheologic behavior, and this also would allow to speculate that both larval stages could seize it from the cells of the host in order to interfere and/or evade the immune response.

Keywords : Trichinella spiralis; Desialylation; Polybrene Method.