Abstract

ARISTIMUNO, Ana María et al. Cross-reactivity of a screening test for celiac disease with other autoantibodies. Acta bioquím. clín. latinoam. [online]. 2017, vol.51, n.1, pp.29-35. ISSN 0325-2957.

The goal of this study was to show the presence of cross-reactivity screening test htTG/DGP for celiac disease (CD) with other autoantibodies present in high titers in different autoimmune diseases (AD). A cross-sectional study was performed for which 100 patients of both sexes (15 men, 85 women), aged between 4 and 86 years without CD who had different autoimmune pathologies were selected. To study the presence of CD, QUANTALite® (INOVA Diagnostics, USA): htTG/DGP Screen, htTG IgA and IgG, Gliadin IgAII and IgGII tests by ELISA were used. Other autoantibodies from AD were determined by indirect immunofluorescence and by electrochemiluminescence. Cross-reactivity with non-specific autoantibodies found in EC was 2.0%. The two positive samples of screening test (23,0 U and 24,9 U) had anti-centromere antibodies 1/1280 and anti-nuclear antibodies 1/640 respectively. They were analyzed for celiac disease markers and only one was weak positive (21,8 U) for anti-Gliadin IgAII. The low cross reactivity found with screening test htTG/DGP in the presence of other autoantibodies made it possible to conclude that this test is a useful tool for screening of CD in patients with different autoimmune diseases.

Keywords : Autoantibodies; Celiac disease; Screening test.