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Acta bioquímica clínica latinoamericana

On-line version ISSN 1851-6114

Abstract

ANGELERI, Anabela et al. Comparison of manual microscope method and an automated cell count in serous fluids. Acta bioquím. clín. latinoam. [online]. 2017, vol.51, n.1, pp. 37-44. ISSN 1851-6114.

The purpose of this work was to compare the total cell count in liquids serous cavities between the automated method used in hematology and the manual method hemocytometer. A total of 107 samples were processed: 45 ascites fluids (LA for its name in Spanish) and 62 pleural fluids (LP for its name in Spanish). The cells were counted in improved Neubauer counting chamber and hematology analyzer Sysmex XT 1800i. The following results were obtained: 1) Linear Regression correlation coefficients indicated a high correlation between the two methods (LA r2: 0.999; p<0.0001 LP r2: 0.997; p<0.0001). 2) Bland-Altman analysis graphics showed excellent agreement between both methods. The systematic error was 51 for LA and 97 for LP; these values are insignificant considering the diagnostic value of the data. he results demonstrate that the methods are comparable and therefore can replace the manual counting by the automated method with proven efficiency and accuracy. However, all fluids require observation by optical microscope before being processed by the hematology analyzer, where the presence of cell clusters such as neoplastic cells in glandular disposition will be appreciated, which hinder the analysis by the equipment or interpretation of results.

Keywords : Serous fluids; Manual microscope cell count method; Automated cell count method; Pleural effusion; Ascitic effusion.

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