Abstract

ASSUM, Débora Magalí; OROZCO, Maria Belén; TANTUCCI, Leonela Ana  and  SUAREZ, Héctor Andrés. Levetiracetam determination in human serum by liquid chromatography with diode array detection. Acta bioquím. clín. latinoam. [online]. 2017, vol.51, n.1, pp.53-61. ISSN 0325-2957.

A new analytical method for Levetiracetam (LEV) determination in human serum was developed and validated by high performance liquid chromatography (HPLC) with diode detection. It is a simple methodology that can be included in the laboratory routine and can be useful in both therapeutic drug monitoring and emergencies. The drug extraction is performed through a liquid-liquid extraction with methyl chloride. Subsequently, the organic phase is evaporated, reconstituted with the mobile phase, and injected in the chromatograph to be detected at 205 nm. LEV retention time is 5 min and it does not show interference with respect to other drugs commonly prescribed with Levetiracetam. The work curve showed linearity between 5.2 and 82.9 μg/mL and a detection and quantification limit of 0.8 μg/mL and 2.7 μg/mL, respectively, while the recovery was of 99.8%.

Keywords : Levetiracetam; Anticonvulsant; High performance liquid chromatography; Analytical method; Validation.