Abstract

BOVONE, Nora Silvia; GASTIAZORO, Ana María  and  FUENTE, María Cristina. Estimation of the reference range for C3 and C4 in a hospital adult population of Greater Buenos Aires. Acta bioquím. clín. latinoam. [online]. 2017, vol.51, n.2, pp.177-181. ISSN 0325-2957.

The aim of this study was to estimate the reference interval for the components C3 and C4 complement in an adult population. The guidelines of the C28A3 document CLSI were followed, for which blood donors attending to a hospital blood centre who passed the medical examination and agreed to extraction were chosen as reference population. A sample constituted by 251 donors, 72.9% women, average age 36.5±10 years and 27.1% men, average age 40.6±11.5 years was selected. This composition was balanced by gender and average age to the population of patients attending the laboratory, mostly with autoimmune diseases. The analytes were measured by rate nephelometry with Immage 800 Nephelometer, Beckman Coulter (California, USA). The reference range for the non-parametric method was calculated, this is to say, the 95% central confidence interval of each value distribution was estimated. The limits obtained were: 95% CI C3=70-165 mg/dL and 95% CI C4=14-37 mg/dL. These results were later verified with a series of 20 new donors and are comparable to values obtained in other studies cited in the literature.

Keywords : C3 complement; C4 complement; Reference interval; Hospital adult population.

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