Abstract

PONCE DE LEON, Patricia  and  LOPEZ MURUA, Gabriel. Erythrocyte desialylation kinetics produced by living Trichinella spiralis muscle larvae. Acta bioquím. clín. latinoam. [online]. 2017, vol.51, n.2, pp.237-242. ISSN 0325-2957.

T. spiralis muscle larvae (ML) alter erythrocyte aggregation. The objective of this work was to study erythrocyte desialylation kinetics produced by living T. spiralis ML. Three experiments were conducted in which 60 larvae were incubated with 30 μL of erythrocytes in 1 mL of saline solution for 1, 2, 3, 4, 5, 6, 7, 10, 15, 18, 20, 22 and 24 hours. Titration of Aggregation by Polybrene Method was used and Title, Total Score and CexpCASP were calculated in Control erythrocytes and erythrocytes incubated with ML. The results showed that in the first hour there was no capture of sialic acid. The coefficient began to decrease at 2 hours, and at 3 hours the Title decreased in one dilution and the coefficient was 0.62±0.021. The title was maintained at the following times and the coefficient value presented small decreases, until reaching 0.45±0.010 value at 22 hours. It was then that, a significant decrease in Title occurred. Within 24 hours, there was a further decrease of Control Title, and CexpCASP was 0.13±0.093. It can be concluded that living ML during in vitro incubation with erythrocytes began to capture sialic acid after 2 hours of contact, getting almost complete desialylation of erythrocytes at 24 hours.

Keywords : Kinetics; Desialylation; Muscle larvae; Trichinella spiralis.

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