Abstract

MUZA BRISENO, Carlos et al. Search for biomarkers from peptides expressed in breast cancer biopsies. Acta bioquím. clín. latinoam. [online]. 2017, vol.51, n.4, pp.621-628. ISSN 0325-2957.

Breast cancer (BC) is one of the leading causes of death in Mexico. Moreover, BC is the main cause of death in women between 15-29 years old in northern Mexico. Proteomic techniques have been used in order to achieve a better understanding of the genes involved in the development of BC. The proteins in BC extracted from 50 fresh breast cancer tissues (FBCT), 50 paraffin embedded breast cancer tissues (PEBCT) and 10 biopsies from women suspected of cancer (SC), residing in Coahuila, Mexico were analyzed in this paper. The quantity of protein extracted was similar in both samples FBCT and PEBCT. However, protein quality was lower in PEBCT than FBCT. Subsequently, these proteins were resolved in SDS-PAGE and 2DE. Differences were noticed in protein profile and all those suspect proteins were analyzed by LC/MS/MS. Amino acidic fingerprint allowed for the identification of peptides associated with a) cell migration, b) tumor suppression, c) oxidative stress or heat shock.

Keywords : Breast cancer; Proteomics; Sodium polyacrylamide-dodecyl sulfate gels in two dimentions; Liquid chromatography coupled to tandem mass spectrometry; Biomarkers; Câncer de mama; Proteômica; Géis de poliacrilamida-dodecil sulfato de sódio; Cromatografia líquida acoplada a massas em tandem; Biomarcadores.

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