Abstract

GOEDELMANN, Carolina Juliana et al. Influence of the EDTAK² and EDTAK³ anticoagulants on hematology testing results. Acta bioquím. clín. latinoam. [online]. 2018, vol.52, n.3, pp.323-330. ISSN 0325-2957.

The International Council for Standardization in Haematology (ICSH) currently supports de use of ethylenediaminetetraacetic acid dipotassium (EDTAK2) anticoagulant for hematology testing, while the Clinical and Laboratory Standards Institute (CLSI) recommends EDTAK2 or ethylenediaminetetraacetic acid tripotassium (EDTAK3). The objective of this study was to evaluate the influence of the EDTA formulations in the results of hematologic parameters. A total of 24 samples were collected by venous puncture and two aliquots were loaded: one in EDTAK3 and another in EDTAK2. Of the 23 hematologic parameters tested with the use of Sysmex XN1000 (Roche diagnostics) hematologic analyser, only those in the red series showed statistically significant differences in the paired samples (p < 0.05). The bias exceeded the quality specifications of Minimum Biological Variability for hematocrit (2.9%) and mean corpuscular hemoglobin concentration (1.7%), Desirable Biological Variability for the mean corpuscular volume (1.7%) and Biological Variability Optimal for hemoglobin (1.2%) and erythrocyte count (1.2%). No significant differences were found in the parameters of white series, neither platelets, nor reticulocytes. To reduce the preanalytical error, each laboratory should standardize the extraction for complete blood cell count using a single type of anticoagulant.

Keywords : EDTA anticoagulant; Complete blood cell count; Pre-analytic phase.

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