SciELO - Scientific Electronic Library Online

 
vol.52 número3Frecuencia de fenotipos del sistema Rh en donantes voluntarios de sangreVaginosis bacteriana en embarazadas y su impacto en la prematurez y en el bajo peso al nacer índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

  • No hay articulos citadosCitado por SciELO

Links relacionados

Compartir


Acta bioquímica clínica latinoamericana

versión impresa ISSN 0325-2957versión On-line ISSN 1851-6114

Resumen

CARDENAS DELGADO, Víctor Manuel; NUNEZ, Myriam  y  NUNEZ TAQUIA, Guillermo Gabriel. Validation of solid phase immunoassays. Acta bioquím. clín. latinoam. [online]. 2018, vol.52, n.3, pp.339-345. ISSN 0325-2957.

Determination of the levels of specific antibodies by semiquantitative ELISAs requires the construction of a 4 parameter logistic regression (4PL) calibration curve. However, alternative models are often employed to adjust and estimate calibration data. The aims of this work were to determine the model that best adjusts calibration data, and which alternative model generates a lower relative percentage error (RPE) in the prediction of calibrator concentrations. An IgA anti-gliadin ELISA was used. The optical density values (OD) of calibrators were adjusted with the following mathematical models: i) Exponential, ii) Boltzmann, iii) Boltzmann semilog, iv) Deming, v) Linear regression, vi) 4PL, and vii) Quadratic. Results indicated that the best adjustment is given by models v) (R2=0.9914), i) (R2=0.9652) and iii) (R2=0.9650). However, models i) and iii) performed better in the reciprocal adjustment procedure: RPE values were ≤20% for all the calibrator levels analyzed (0-100 IU/mL). The linear models iv) and v) had high RPE values. To sum up, the exponential (i) and Boltzmann (iii) adjustments present R2 and RPE values similar to those of the 4PL, the use of any linear adjustment being inappropriate.

Palabras clave : Calibration; Semiquantitative ELISA; Immunoassay; Logistic model; Linear model; Validation; Comparison; Reciprocal adjustment; Relative error; Estimation.

        · resumen en Español | Portugués     · texto en Español     · Español ( pdf )

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons