SciELO - Scientific Electronic Library Online

 
vol.53 issue1Efecto de Trichinella spiralis y de Trichinella patagoniensis n.sp. sobre la desializaciónConocimiento sobre infecciones asociadas a la atención de la salud en estudiantes de bioanálisis author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

  • Have no cited articlesCited by SciELO

Related links

Share


Acta bioquímica clínica latinoamericana

Print version ISSN 0325-2957On-line version ISSN 1851-6114

Abstract

MARTINEZ-CABRERA, Ileana et al. Una vacuna de vesículas de membrana externa de Vibrio cholerae es aún una promesa: Caracterización proteómica. Acta bioquím. clín. latinoam. [online]. 2019, vol.53, n.1, pp.43-51. ISSN 0325-2957.

Cholera epidemics affect a large number of African, Asian and Caribbean countries. The climate changes and the constant migrations cause this disease to spread, making it is necessary to obtain protective vaccines. In the present work, a new vaccine of outer membrane vesicles (OMV) from V. cholerae O1 El Tor biotype Ogawa serotype strain C7258 at Finlay Institute of vaccines (Cuba) was characterized by proteomic methods. A total of 53 proteins present in the OMV (approximate ratio of 4 proteins by electrophoresis band) were identified, separated by one dimension electrophoresis and digested by tripsin method. The fragments were separated by high performance liquid chromatography (HPLC) coupled to mass spectrometry, sequenced and identified, using Swiss-Prot and TrEMBL protein databases. The pattern showed some proteins (12 cytoplasmic proteins and 5 outer membrane proteins) suggested within the highest quality proteome for vaccine candidate. The best conditions for proteins separation by two dimension electrophoresis were studied. The OMV composition was based on proteins described to the immunity response and protection against V. cholerae O1 El Tor biotype Ogawa serotype.

Keywords : Vibrio cholerae; Proteomics; Vaccines; Outer membrane proteins.

        · abstract in Spanish | Portuguese     · text in Spanish     · Spanish ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License