Abstract

ZUNIGA-SOSA, Evelyn Alexandra; PULLAS-BAHAMONDE, María Fernanda; CRESPO-PROANO, Carolina Elizabeth  and  CHIRIBOGA-PONCE, Rosa F. Tipificación del antígeno Duffy como método indirecto para identificar individuos asintomáticos de malaria. Acta bioquím. clín. latinoam. [online]. 2019, vol.53, n.1, pp.71-77. ISSN 0325-2957.

Despite the strategies carried out annually for the control and eradication in countries considered endemic for malaria, there are isolated outbreaks probably due to asymptomatic carriers. These involve the presence of individuals with Duffy negative phenotype or population movements. The neighbourhood “50 casas” of the province of Esmeraldas-Ecuador has all these characteristics, so the purpose of this research was to determine the presence of the Fy(a-b-) phenotype and its relationship with the Plasmodium vivax. A survey was carried out into each member of the participating families and after accepting and signing the informed consent, blood samples were taken to perform serological tests in search of antibodies and antigens of P. vivax. The phenotyping of Duffy system and real-time PCR tests for DNA determination of Plasmodium vivax and falciparum was realized. The results showed that there is a statistically significant association between the Duffy phenotype and P. vivax malaria (p<0.05). In view of this situation, the control agencies should propose new strategies for the detection of asymptomatic carriers and preventive measures to hinder new outbreaks of malaria and determine if P. vivax has found a new invasion mechanism in individuals with the Fy(a-b-) phenotype.

Keywords : Duffy; Malaria; Phenotyping; Plasmodium vivax.

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