Revista argentina de microbiología
versión On-line ISSN 1851-7617
BIDINOST, C. et al. Virulence factors of non-O1 non-O139 Vibrio cholerae isolated in Córdoba, Argentina. Rev. argent. microbiol. [online]. 2004, vol.36, n.4, pp. 158-163. ISSN 1851-7617.
V. cholerae non-O1 non-O139 serogroups isolated from clinical and environmental sources in Córdoba, Argentina, were analyzed for the presence and expression of virulence genes. Most of the strains studied contained the genes toxR and hlyA, but lacked ctxA, zot, ace, tcpA and stn. The culture supernatants were tested for hemolytic and cytotoxic activity. The enterotoxic potential of the strains was studied in a rabbit ileal loop assay and their genetic profiles were compared by PFGE. The environmental strains varied in their virulence phenotype and showed no-clonal relationships. The clinical strains were highly enterotoxic, hemolytic, proteolytic and showed indistinguishable PFGE profiles, although they differed in their cytotoxic activity. This is the first description, using cell culture and “in vivo” studies, of the virulence properties of non-O1 non-O139 V. cholerae from Argentina.
Palabras clave : non-O1 non-O139 V. cholerae; enterotoxin; cytotoxin; hemolysin; multiplex PCR.