Abstract

GIACOBONI, G.; ECHEVERRIA, M.G.  and  PERFUMO, C.. PCR-RFLP for Campylobacter jejuni subtyping. Rev. argent. microbiol. [online]. 2005, vol.37, n.2, pp.81-83. ISSN 0325-7541.

Ten Campylobacter jejuni isolates, 8 identified as C. jejuni biotype II of Lior and 2 as C. jejuni biotipe I, were recovered from aborted pig fetuses. In order to discriminate among strains, restriction fragment length polymorphism (RFLP) using DdeI of polymerase chain reaction (PCR) products of flaA gen was used. C. jejuni biotype II strains could be diferenciated in 6 by PCR-RFLP, and one subtype was obtained from C. jejuni biotype I. Although there is great variability of molecular techniques applied to the Campylobacter epidemiological studies, PCR-RFLP demonstrated to be a simple and accessible technique to discriminate Campylobacter jejuni isolates.

Keywords : Campylobacter jejuni; subtypes; PCR-RFLP.

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