Revista argentina de microbiología
versión On-line ISSN 1851-7617
FUSELLI, S. R. et al. Inhibition of Paenibacillus larvae employing a mixture of essential oils and thymol. Rev. argent. microbiol. [online]. 2006, vol.38, n.2, pp. 89-92. ISSN 1851-7617.
In vitro antimicrobial activity of a mixture of two essential oils and thymol against Paenibacillus larvae, causal agent of American Foulbrood (AFB), was evaluated. The essential oils were extracted from cinnamon (Cinnamomum zeylanicum) and thyme (Thymus vulgaris). The third component used, thymol, is the major component of the essential oil of thyme which contains 39.9 % of thymol. Minimal inhibitory concentration (MIC) in Mueller-Hinton broth by the tube dilution method and minimal bactericide concentration (MBC) on MYPGP agar were evaluated. Thyme registered MIC values of 150-250 μg/ml and MBC values of 200-300 μg/ml, while the MIC and MBC values obtained for cinnamon were of 50-100 μg/ml and 100-125 μg/ml. Thymol showed similar MIC and MBC values of 100-150 μg/ml. No significant differences between the bacterial strains were detected, but significant differences between essential oils and thymol activity were registered (P<0,01). An inhibitory synergetic effect on AFB was observed reducing MIC and MBC values due to the use of a mixture of 62.5% of thyme, 12.5% of cinnamon and 25% of thymol.
Palabras clave : essential oils; Cinnamomum zeylanicum; Thymus vulgaris; thymol; Paenibacillus larvae.