Revista argentina de microbiología
versión On-line ISSN 1851-7617
BRACESCO, N. et al. Analysis of a putative protection against free radicals by grape derivatives (Vitis vinifera L. Cv. Tannat) in Saccharomyces cerevisiae. Rev. argent. microbiol. [online]. 2007, vol.39, n.1, pp. 4-10. ISSN 1851-7617.
The aim of this work was to analyse a possible genome protection provided by a grape derivative (Tannat wine) in yeast cell populations exposed to H2O2. Haploid and diploid strains of Saccharomyces cerevisiae were used as eukaryotic model. Cell samples were exposed to H2O2 in a nutrient medium. Chromosomal DNA was analysed after isolation and separation by pulsed field electrophoresis. Double strand breaks were determined by laser densitometry and application of Poisson distribution. Both haploid and diploid cells showed H2O2 dose dependent DNA fractionation, as well as an increase of lethal -and mutation- events. Upon combination of the Tannat wine and H2O2 a significant decrease of double strand breaks was observed, in association with an increase in surviving fractions. No mutagenic effect was observed after wine exposure. Part of the observations regarding protective wine effect were simulated by exposure to high concentrations of α-tocopherol. Present results indicate that a grape derivative could act as a genome protector increasing cell survival probabilities. Among others, the involved molecular targets could be components of transduction redox cascades as well as DNA repair enzymes.
Palabras clave : peroxide; Tannat wine; genome; Saccharomyces cerevisiae.